Journal: Molecular Cancer Therapeutics

Article Title: Preclinical Profile of BYON3521 Predicts an Effective and Safe MET Antibody–Drug Conjugate

doi: 10.1158/1535-7163.MCT-22-0596

Figure Lengend Snippet: Competition experiments with rhHGF and MET ECD. A, BLI experiment to assess competition of rhHGF for MET ECD binding of SYD2884. Presented are the binding curve of SYD2884 visualized with an HRP-conjugated anti-hIgG (red line, solid squares) and rhHGF visualized using a polyclonal anti-HGF antibody (blue line, solid triangles). The detectable amount of SYD2884 binding to the recombinant human MET ECD, immobilized on the surface of the sensor tip, decreases whereas at the same time the detectable amount of rhHGF increases. The data are presented as the correlation between the measured signals versus the molar excess of fully activated rhHGF (left graph) and pro-HGF (right graph). B, Cell viability of EBC-1 cells treated with increasing concentrations of BYON3521 or nonbinding isotype control ADC in the presence or absence of 1 μg/mL MET ECD, either administered together directly, or after 30-minute preincubation of BYON3521 or isotype control ADC and MET ECD (Premix). (Approximate MW of BYON3521 is 149 kD, of SY2884 144 kD, and MET ECD 140 kD).

Article Snippet: To evaluate the potential interference of HGF with cellular binding, cells were preincubated with 320 ng/mL recombinant human (rh)HGF (30 minutes at 4°C; Sino Biological; mixture of pro-HGF and activated HGF) prior to incubation with antibody or ADC.

Techniques: Binding Assay, Recombinant

Journal: Journal of Clinical Medicine

Article Title: A Novel Bioengineered Functional Motor Unit Platform to Study Neuromuscular Interaction

doi: 10.3390/jcm9103238

Figure Lengend Snippet: Complete growth media for skeletal muscle cell proliferation.

Article Snippet: Recombinant human hepatocyte growth factor (HGF) from Sino Biological Inc. (Beijing, China) , 2.5 ng/mL , 10463-HNAS.

Techniques: Concentration Assay, Modification, Recombinant

Journal: Scientific Reports

Article Title: Autophagy inhibition enhances antifibrotic potential of placental mesenchymal stem cells of fetal origin via regulating TGF-β1 mediated protein degradation of HGF

doi: 10.1038/s41598-025-97054-8

Figure Lengend Snippet: Effects of HGF regulated by autophagy on antifibrotic function of fPMSCs. The conditioned medium from sh-NC and sh-ATG5 infection of fPMSCs were infected with sh-NC and sh-ATG5 lentivirus and pretreated with 20 ng/mL TGF-β1 in order to prepare conditioned medium, then which was conducted to culture MRC-5 cells stimulated with 2.5 ng/mL TGF-β1 for 24 h. 500 ng/mL recombinant human HGF (rhHGF) and 1 μg/mL HGF neutralization antibody (naHGF) was added into conditioned medium respectively to interrupt the function of HGF. Protein levels of Collagen I, α-SMA and CTGF were detected by western blotting. Densitometric analyses of Collagen I, α-SMA and CTGF. Representative data from three independent experiments are shown. * P < 0.05.

Article Snippet: A coculture system was treated with 500 ng/mL recombinant human HGF (Proteintech, China) and 1 μg/mL HGF neutralization antibody (R&D Systems, USA) to verify the antifibrotic function of HGF derived from fPMSCs on MRC-5 cells.

Techniques: Infection, Recombinant, Neutralization, Western Blot

Journal: Experimental Biology and Medicine

Article Title: Mesenchymal stem cells alleviate hypoxia-induced oxidative stress and enhance the pro-survival pathways in porcine islets

doi: 10.1177/1535370219844472

Figure Lengend Snippet: IL-6 plays a role in MSCs' protection. (a) ELISA quantification of IL-6 and HGF in MSC-CM. (b) The recombinant human IL-6 (rIL-6) decreased the hypoxia-induced death of NICCs. rIL-6 suppressed total ROS accumulation (c) and augmented protein phosphorylation (d) under hypoxic conditions. nor: normoxia; hypo: hypoxia; CM: conditioned medium. Values are means ± SD. **P < 0.01, ***P < 0.001. (A color version of this figure is available in the online journal.)

Article Snippet: Human IL6 ELISA Kit (Proteintech, USA) and human hepatocyte growth factor (HGF) ELISA kit (CUSABIO, China) were used to detect the concentration of IL-6 and HGF in MSC-CM, respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Phospho-proteomics

Journal: American Journal of Translational Research

Article Title: Portal modulation effects of terlipressin on liver regeneration and survival in a porcine model subjected to 90% hepatectomy

doi:

Figure Lengend Snippet: Postoperative change in serum levels of interleukin (IL)-6 (A), hepatocyte growth factor (HGF) (B), and endothelin-1 (ET-1) (C) evaluated using enzyme-linked immunosorbent assay. Data are expressed as the median, with the 25-75% percentiles in boxes and the 5-95% percentiles as whiskers. *P < 0.05 vs. control group at the same time point.

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) Interleukin 6 (IL-6), hepatocyte growth factor (HGF), and endothelin-1 (ET-1) serum levels were measured using commercially available ELISA kits (IL-6; Porcine IL-6 Quantikine ELISA Kit, P6000B, R&D systems, USA) (HGF; Pig hepatocyte growth factor, HGF ELISA Kit, CSB-E06795p, CUSABIO, China) (ET-1; Endothelin-1 Quantikine ELISA Kit, DET100, R&D systems, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Scientific Reports

Article Title: Autophagy inhibition enhances antifibrotic potential of placental mesenchymal stem cells of fetal origin via regulating TGF-β1 mediated protein degradation of HGF

doi: 10.1038/s41598-025-97054-8

Figure Lengend Snippet: TGF-β1 suppresses HGF expression in fPMSCs. Proteomics of the condition medium from fPMSCs treated with or with 20 ng/mL TGF-β1, volcano plot ( A ) and Venn diagram ( B ) revealed 146 upregulated and 159 downregulated proteins, with statistical significance. ( C ) Heatmap of proteomic analysis showing HGF downregulation in the extracellular region (GO:0005576). ( D ) Western blot analysis of HGF protein expression in fPMSCs treated with increasing TGF-β1 concentrations (0–50 ng/mL). ( E ) ELISA quantification of HGF secretion. Data are representative of three independent experiments. * P < 0.05.

Article Snippet: According to the manufacturer’s instructions, human HGF ELISA kits (BOSTER, China) were used to measure the concentrations of HGF secreted by fPMSCs.

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Autophagy inhibition enhances antifibrotic potential of placental mesenchymal stem cells of fetal origin via regulating TGF-β1 mediated protein degradation of HGF

doi: 10.1038/s41598-025-97054-8

Figure Lengend Snippet: Effect of TGF-β1 reduces HGF expression dependent on autophagy in fPMSCs. fPMSCs were infected with sh-NC and sh-ATG5 lentivirus, and treated with or without TGF-β1 (20 ng/mL). Expression of HGF, LC3-I/II, and ATG5 were assessed by Western blotting ( A ). Secretion levels of HGF in each group were quantified through an ELISA assay ( B ). The interaction between p62 and HGF in TGF-β1-treated fPMSCs was examined via a Co-IP assay ( C ). NC represents the negative control. Representative data from three independent experiments are shown. * P < 0.05.

Article Snippet: According to the manufacturer’s instructions, human HGF ELISA kits (BOSTER, China) were used to measure the concentrations of HGF secreted by fPMSCs.

Techniques: Expressing, Infection, Western Blot, Enzyme-linked Immunosorbent Assay, Co-Immunoprecipitation Assay, Negative Control